Effect of apigenin, kaempferol and resveratrol on the expression of interleukin-1 and tumor necrosis factor- genes in J774.2 macrophages
نویسندگان
چکیده
Flavonoids have been reported to bring benefits in lowering inflammation, oxidative stress and exert positive effects in cancer and cardiovascular and chronic inflammatory diseases. Apigenin, kaempferol and resveratrol present in fruits, vegetables and grain were investigated for their effect on the synthesis of interleukin-1 (IL-1 ) and tumor necrosis factor(TNF) at transcriptional level in lipopolysaccharide (LPS)-stimulated J774.2 macrophages. Apigenin (30 M), kaempferol (30 M) and resveratrol (50 M) significantly decreased the number of TNFmRNA copies in LPS-activated J774.2 macrophages. Apigenin and kaempferol caused inhibition of IL-1 gene expression in J774.2 macrophages, but resveratrol was ineffective. These results indicate that apigenin, kaempferol and resveratrol exert inhibitory effects on the TNFand except for of resveratrol on IL-1 gene expression in J774.2 macrophages at the transcriptional level. In addition, the studied compounds may be the mediators responsible for protective role of a diet high in fruits and vegetables in the cardiovascular and inflammatory diseases.
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